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目的 :检测喉癌组织TGF β1的表达 ,分析TGF β1与喉癌临床指标的关系 ,明确TGF β1表达对TIL细胞浸润和局部免疫功能的影响。方法 :38例原发性喉癌标本 ,应用免疫组化SP法 ,检测喉癌组织CD3 、CD4 、CD8、CD57、CD68和CD2 5的浸润及TGF β1的表达 ;同时以 10例声带息肉做对照组。结果 :与对照组相比 ,喉癌组织TGF β1蛋白表达减少 ,且TGF β1表达与喉癌病人性别、年龄、临床分期及不同发生部位无关 ,随着恶性度的增高 ,TGF β1表达减少 ,淋巴结转移组TGF β1阳性率多于未转移组 ,TGF β1表达与CD4 细胞浸润无关 ,而与CD8浸润有显著正相关 ,TGF β1表达能诱发CD4/CD8比值倒置。结论 :喉癌组织TGF β1蛋白表达减少 ,TGF β1对喉癌细胞的生长可能起抑制作用 ,有利于肿瘤细胞的转移 ,通过影响CD8细胞的浸润 ,改变CD4/CD8比值 ,进而抑制肿瘤局部免疫功能。
OBJECTIVE: To detect the expression of TGFβ1 in laryngeal carcinoma, analyze the relationship between TGFβ1 and clinical characteristics of laryngeal carcinoma, and to clarify the effect of TGFβ1 expression on TIL infiltration and local immune function. Methods: Thirty-eight cases of primary laryngeal carcinoma were examined by immunohistochemistry SP method to detect the infiltration of CD3, CD4, CD8, CD57, CD68 and CD25 and the expression of TGF-β1 in laryngeal carcinoma. group. Results: Compared with the control group, the expression of TGF-β1 in laryngeal carcinoma was decreased, and the expression of TGF-β1 was not correlated with the gender, age, clinical stage and different sites of laryngeal carcinoma. With the increase of malignancy, the expression of TGF-β1 decreased, The positive rate of TGF-β1 in metastatic group was higher than that in non-metastatic group. The expression of TGF-β1 was not related to the infiltration of CD4 cells, but positively correlated with the infiltration of CD8. The expression of TGF-β1 could induce the up-regulation of CD4 / CD8 ratio. CONCLUSION: The expression of TGF-β1 in laryngeal carcinoma is decreased. TGF-β1 may inhibit the growth of laryngeal carcinoma cells and promote the metastasis of tumor cells. By affecting the infiltration of CD8 cells and changing the ratio of CD4 / CD8, TGFβ1 may inhibit the local immune function .