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目的 :探讨HIV - 1TAT的蛋白转导结构域 (proteintransductiondomain ,PTD)介导的大分子蛋白通过血脑屏障 (blood -brainbarrier,BBB)的作用及其在脑组织中的分布。方法 :将纯化的PTD -BCR/ABL融合蛋白及伊文思蓝经尾静脉注入小鼠体内 ,制备全脑组织恒冷箱连续切片 ,用免疫组织化学染色法检测PTD -BCR/ABL融合蛋白在脑组织中的分布 ,用伊文思蓝作为指示剂检测PTD对BBB开放的影响。结果 :将其注射到小鼠体内后 ,在全脑组织细胞中均检测到PTD -BCR/ABL融合蛋白 ,呈非特异性组织分布 ;全脑组织除无BBB区域外 ,余脑区均无伊文思蓝渗入 ,PTD不引起BBB的开放。结论 :PTD可在体内特异性介导大分子蛋白通过血脑屏障 ,并在全脑组织细胞中分布 ,为肽类或蛋白类大分子药物通过BBB进入中枢神经系统 (cen tralnervousystern ,CNS)开辟了一条新的途径。
Objective: To investigate the role of protein - protein transduction domain (PTB) mediated by HIV - 1 TAT in the blood - brain barrier (BBB) and its distribution in brain tissue. Methods: Purified PTD-BCR / ABL fusion protein and Evans blue were injected into mice via the tail vein to prepare serial sections of total brain tissue in a constant temperature refrigerator. Immunohistochemical staining was used to detect the expression of PTD-BCR / ABL fusion protein in brain Tissue distribution was assessed using Evans blue as an indicator for the effect of PTD on BBB opening. Results: The PTD-BCR / ABL fusion protein was detected in whole brain tissue after injection into mice, showing non-specific tissue distribution. In the whole brain tissue, there were no Evans Blue infiltration, PTD does not cause BBB open. CONCLUSION: PTD can specifically mediate macromolecular protein cross-flow through the blood-brain barrier in vivo and distribute in whole brain tissue cells, opening up the entry of peptides or protein macromolecules into the cen tralnervous system (CNS) via the BBB A new way.