论文部分内容阅读
AIM:To explore the correlation of the inherent cellular ROSlevel with the susceptibility of the digestive tract tumor cellsto apoptosis inducted by As_2O_3.METHODS:Two gastric cercinoma cell lines,SGC7901 andMKN45,and two esophageal carcinoma cell lines,EC/CUHKI(alternatively named EC1.71)and EC1867 with lowconcentration(2μmol·L~(-1))of As_2O_3 were cultured respectly,which confirmed the difference in apoptosis susceptibilitybetween SGC7901 and MKN45,and between EC/CUHK1 andEC1867.The cells were incubated withdihydrogenrhodamine123(DHR123),used as a ROScapture,in absence of As_2O_3.The fluorescent intensity ofrhodamine123,which was the product of cellular oxidationof DHR123,was detected by flow cytometry,and ROS wasmeasured.RESULTS:Apoptosis induced by a low concentration ofAs203 was more readily to occur in SGC7901(22.4%±2.4%)and EC/CUHK1(27.0%±2.9%)than in MKN45(2.1%±0.5%)and EC1867(0.8%±0.5%).In other words,SGC7901was more sensitive than MKN45 to As_2O_3,meanwhile EC/CUHK1 was more sensitive than EC1867 to As_2O_3.The levelof inherent cellular ROS in SGC7901(650±37)was higherthan that in MKN45(507±22)(P<0.01),and the level ofinherent cellular ROS in EC/CUHK1(462±17)was higherthan that in EC1867(187±12)(P<0.01).CONCLUSIONS.The cellular sensitivity to apoptosis inducedby As_2O_3 is associated with the difference in cellular ROSlevel.The inherent ROS level might determinate theapoptotic sensitivity of tumor cells to As_2O_3.
AIM: To explore the correlation of the inherent cellular ROSlevel with the susceptibility of the digestive tract tumor cellsto apoptosis induced by As_2O_3.METHODS: Two gastric cercinoma cell lines, SGC7901 and MKN45, and two esophageal carcinoma cell lines, EC / CUHKI (alternatively named EC1 .71) and EC1867 with low concentration (2μmol·L -1) of As_2O_3 were cultured respectly, which confirmed the difference in apoptosis susceptibilitybetween SGC7901 and MKN45, and between EC / CUHK1 andEC1867.The cells were incubated with dihydrogenrhodamine 123 (DHR123) used as a ROScapture, in absence of As_2O_3. The fluorescent intensity of rhodamine123, which was the product of cellular oxidation of DHR123, was detected by flow cytometry, and ROS wasmeasured.RESULTS: Apoptosis induced by a low concentration of As203 was more readily to occur in SGC7901 (22.4% ± 2.4%) and EC / CUHK1 (27.0% ± 2.9%) than in MKN45 (2.1% ± 0.5%) and EC1867 meanwhile EC / CUHK1 was more sensitive than EC1867 to As_2O_3.the level of inherent cellular ROS in SGC7901 (650 ± 37) was higherthan that in MKN45 (507 ± 22) (P <0.01), and the level of intrinsic cellular ROS in EC / CUHK1 was higherthan that in EC1867 (187 ± 12) (P <0.01). CONCLUSIONS. The cellular sensitivity to apoptosis induced by As_2O_3 is associated with the difference in cellular ROS level. The inherent ROS level might determinate the apoptotic sensitivity of tumor cells to As_2O_3.