论文部分内容阅读
以灰霉菌毒素为选择剂,利用多步筛选法筛选番茄抗性愈伤组织突变体,结果显示,毒素对番茄愈伤组织的诱导、生长具有抑制作用,且随毒素浓度的升高而增强;获得的抗性愈伤组织在不同浓度毒素胁迫下,其增殖率均高于原始型愈伤组织,并在离开选择因子继代培养2代后,仍保持获得的抗性。研究毒素处理后过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)的活性变化发现,无论有无毒素胁迫,抗性愈伤组织突变体及其再生植株的酶活性均高于原始型愈伤组织和原始植株(对照);随着毒素胁迫浓度的增大,抗性愈伤组织突变体及再生植株的3种酶活性有相同的变化趋势,即先升高后降低,且均比对照升高幅度大,其中PAL和PPO酶活性的下降速度较对照慢,说明抗性突变体通过增强与抗病性相关的酶活性,增强植物的防卫能力。
The results showed that toxin had inhibitory effect on the induction and growth of tomato callus, and increased with the increase of toxin concentration. Tomato - resistant callus mutants were screened by multi - step screening method. The resistant callus obtained under different concentrations of toxin had higher proliferation rate than that of the original callus, and maintained the acquired resistance after leaving the selection factor subculture for two generations. To study the changes of peroxidase (POD), phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) activity after toxin treatment, we found that mutants with resistant callus and their The enzymatic activities of the regenerated plants were higher than that of the original callus and the original plants (control). With the increase of the toxin stress concentration, the three enzyme activities of the resistant callus mutants and regenerated plants showed the same trend, That is, firstly increased and then decreased, and both increased greatly compared with the control, and the decline rate of PAL and PPO enzyme activities was slower than that of the control, indicating that the resistant mutant enhances the defense ability of plants by enhancing the enzyme activity related to disease resistance .