论文部分内容阅读
采用反相高效液相色谱法测定维生素D2 微粒胶囊中VD2 的含量。用AlltechC18柱 ( 1 5 0mm× 4 6mmID ,3μm) ,流速为 1 0mL·min- 1,流动相为V(甲醇 )∶V(水 ) =90∶1 0 ,以苯并 [e]芘为内标 ,UV2 6 5nm检测 ,本法简便、准确、重现性好[1] [2 ] 。
Determination of VD2 in vitamin D2 capsules by RP-HPLC. The mobile phase was V (methanol): V (water) = 90: 1 0 and benzo [e] pyrene was used as the internal standard in a Alltech C18 column (150 mm × 4 6 mmID, 3 μm) at a flow rate of 10 mL · min -1 Standard, UV2 6 5nm detection, the method is simple, accurate, reproducible [1] [2].