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应用多聚酶链反应（ＰＣＲ）检测５０例大肠腺癌、３８例大肠腺瘤和２０例正常大肠粘膜组织的ＨＰＶ１６型ＤＮＡ序列，并对其扩增产物进行电泳分析和Ｓｏｕｔｈｅｒｎ核酸杂交分析。结果显示，三组病人ＨＰＶ１６型ＤＮＡ阳性率分别为４２％、３１６％和０。大肠腺癌和腺瘤分别与正常大肠粘膜组织比较，ＨＰＶ１６型ＤＮＡ阳性率差异有显著性（Ｐ＜００５）；大肠腺癌和腺瘤之间比较，ＨＰＶ１６型ＤＮＡ阳性率差异无显著性（Ｐ＞００５）；但ＨＰＶ１６型ＤＮＡ阳性的１２例大肠腺瘤组织均伴组织细胞异型增生。结果表明，ＨＰＶ１６型与国人的大肠腺癌有关，是大肠癌的病因之一。
Polymerase chain reaction (PCR) was used to detect the HPV16 DNA sequence of 50 cases of colorectal adenocarcinoma, 38 cases of colorectal adenoma and 20 cases of normal colorectal mucosa. The amplified products were analyzed by electrophoresis and Southern hybridization. The results showed that the positive rates of HPV16 DNA in the three groups were 42%, 31.6%, and 0, respectively. The positive rate of HPV16 DNA in colorectal adenocarcinoma and adenoma was significantly higher than that in normal colorectal mucosa (P<005). There was no significant difference in the positive rate of HPV16 DNA between colorectal adenocarcinoma and adenoma. (P>005); However, 12 cases of HPV16 DNA-positive colorectal adenomas were associated with dysplasia of histiocytes. The results show that HPV16 is associated with colorectal adenocarcinoma in the Chinese population and is one of the causes of colorectal cancer.