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人巨细胞病毒(HCMV)为双链DNA疱疹病毒,在人类有较广泛的分布,一般呈亚临床感染状态,其致炎性、致畸性和致癌性已引起国内外学者的极大关注。目前国内尚未见HCMV致妇科疾病的报道,国外也无分子水平检测HCMV的临床资料。本文于1988年8～11月,首次采用α-~(32)P体外标记的AD169株HCMV—EcoRIZ片段DNA採针对宫颈脱落细胞和宫颈分泌物进行斑点杂交试验,检测HCMV DNA同源序列
Human cytomegalovirus (HCMV) is a double-stranded herpes simplex virus, which is widely distributed in humans and generally presents as a subclinical infection. Its cytotoxicity, teratogenicity and carcinogenicity have attracted great attention of scholars at home and abroad. At present, no reports of gynecological diseases caused by HCMV have been reported in China and clinical data of molecular level for detecting HCMV are not available abroad. This article for the first time in 1988 8 ~ November, the first use of α - (32) P in vitro markers of AD169 strain HCMV-EcoRIZ DNA fragment on cervical exfoliated cells and cervical secretions were spot blot hybridization test HCMV DNA homologous sequences