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目的 研究日本血吸虫 (中国大陆株 )重组信号蛋白 14 3 3 (rSj14 3 3 )作为血吸虫病疫苗分子的潜能 ,并探讨rSj14 3 3、rSjGST两种重组蛋白作为疫苗的协同作用及结核杆菌低分子量耐热多肽 (Mtb)激活的γδ T细胞在抗血吸虫病中的作用。 方法 用SDS PAGE、电洗脱和透析的方法制备rSj14 3 3和rSjGST抗原 ,将两种抗原 (分别用福氏佐剂和Mtb为佐剂 )分别免疫BALB/c小鼠后 ,进行尾蚴攻击感染实验。在攻击感染 6wk后 ,剖杀小鼠计算各组的减虫率。 结果 各组的减虫率为rSj14 3 3 +福氏佐剂组 3 2 .2 0 % ,rSj14 3 3 +rSjGST +福氏佐剂组 3 1.10 % ,rSj14 3 3 +Mtb佐剂组 2 7.96% ,rSj14 3 3 +rSjGST +Mtb佐剂组 2 6.0 0 % ,rSjGST +Mtb佐剂组 2 7.10 % ;各组的减卵率分别为 (按以上组序 ) 5 0 .40 %、5 3 .3 0 %、5 1.10 %、5 8.60 %和 5 1.3 0 %。 结论 rSj14 3 3具有一定的抗血吸虫潜能 ,有可能成为抗日本血吸虫疫苗 ,但未见rSj14 3 3和rSjGST的协同作用 ;Mtb激活扩增的γδ T细胞在抗血吸虫免疫中的效果与福氏佐剂产生的免疫作用类似。
Objective To study the potential of recombinant signaling protein 14 3 3 (rSj14 3 3) of Schistosoma japonicum (Chinese mainland strain) as a vaccine molecule for schistosomiasis and to explore the synergistic effect of rSj14 3 3 and rSjGST as vaccines and the low molecular weight of Mycobacterium tuberculosis Role of Mtb Activated γδ T Cells in Anti-schistosomiasis. Methods The rSj14 3 3 and rSjGST antigens were prepared by SDS PAGE, electroelution and dialysis. BALB / c mice were immunized with two antigens (Adjuvant with Freund’s adjuvant and Mtb respectively) experiment. After 6wk of challenge infection, mice were sacrificed to calculate the worm reduction rate for each group. Results The worm reduction rates of each group were rSj14 3 3 + Freund’s adjuvant group 3.2 2.0%, rSj14 3 3 + rSjGST + Freund’s adjuvant group 3 1.10%, rSj14 3 3 + Mtb adjuvant group 2 7.96% , 2 6.0% of rSj14 3 3 + rSjGST + Mtb adjuvant group and 2. 7.10% of rSjGST + Mtb adjuvant group respectively. The egg reduction rates of each group were (by the above group) 50.40%, 53.3 0%, 5 1.10%, 5 8.60% and 5 1.3 0%. Conclusion rSj14 3 3 has some anti-schistosomiasis potential and may become an anti-Schistosoma japonicum vaccine, but no synergism between rSj14 3 3 and rSjGST is found. The effect of Mtb-activated γδ T cells on anti-schistosome immunity is similar to that of Freund’s The agent produces similar immune effects.