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近年来发现多种miRNA在心血管系统中发挥了极其重要的作用。应用microRNA(miRNA)芯片技术对miRNA在先天性心脏病相关肺动脉高压(pulmonary arterial hypertension,PAH)肺组织内的变化进行研究。收集室间隔缺损(ventricular septal defect,VSD)合并重度PAH(PAH组,n=6)和不伴PAH患者肺组织(对照组,n=6)。分别提取总RNA,然后采用miRNA芯片进行miRNA表达谱差异分析,并对结果进行实时定量PCR验证。和对照组相比,PAH组miRNA表达谱中共有78个差异表达位点,其中有62个上调,16个下调。提示miRNA参与了先天性心脏病继发性肺动脉高压的调节作用,可能成为预防和逆转PAH的新靶点。
In recent years, a variety of miRNAs have been found to play an extremely important role in the cardiovascular system. The microRNA (miRNA) microarray technique was used to study the changes of miRNA in the lung tissue of pulmonary arterial hypertension (PAH) patients with congenital heart disease. Ventricular septal defects (VSD) were collected in severe PAH (PAH group, n = 6) and lung tissue in patients without PAH (control group, n = 6). Total RNA was extracted, and miRNA microarrays were used to analyze miRNA expression profiles, and the results were verified by real-time quantitative PCR. Compared with the control group, there were 78 differentially expressed miRNA expression profiles in PAH group, of which 62 were up-regulated and 16 were down-regulated. These results suggest that miRNA is involved in the regulation of secondary pulmonary hypertension in congenital heart disease and may be a new target for preventing and reversing PAH.