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白细胞介素１（ＩＬ－１）是一种重要的细胞因子，具有广泛的生物学活性。它通过与细胞表面的白细胞介素 １受体（ＩＬ－１Ｒ）结合而起作用。以杆状病毒为载体在昆虫细胞中克隆表达了小鼠Ｉ型可溶性白细胞介素１受体（ｓＩＬ－１ ＲＩ）基因。以ＮＩＨ／３Ｔ３细胞ＲＮＡ为模板，采用ＲＴ－ＰＣＲ方法扩增得到小鼠ｓＩＬ－ＩＲＩ的ｃＤＮＡ，克隆至杆状病毒转移载体ｐＡｃＧＰ６７Ｂ，将转移重组质粒与野生病毒ＡＣＮＰＶ ＤＮＡ共转染昆虫细胞Ｓｆ９，经同源重组得到重组杆状病毒ｒＡＣＮＰＶ。应用经纯化的ｒＡｃＮＰＶ感染昆虫细胞Ｓｆ９，表达获得重组的ｓＩＬ－１ＲＩ。经对亲和层析样品的ＳＤＳ－ＰＡＧＥ分析和对ＩＬ－１β生物活性阻断作用实验证实，表达产物能够与其配基结合，并且能够分泌至细胞培养上清中。
Interleukin-1 (IL-1) is an important cytokine with a wide range of biological activities. It works by binding to the interleukin 1 receptor (IL-1R) on the cell surface. The mouse type I soluble sIL-1 RI gene was cloned and expressed in insect cells using baculovirus as a vector. The cDNA of mouse sIL-IRI was amplified by RT-PCR using NIH / 3T3 cell RNA as a template and cloned into the baculovirus transfer vector pAcGP67B. The recombinant plasmid and the wild-type ACNPV DNA were co-transfected into insect cells Sf9, Recombinant baculovirus rACNPV was obtained by homologous recombination. The purified rAcNPV was used to infect insect cell Sf9, and recombinant sIL-1RI was obtained. SDS-PAGE analysis of the affinity chromatography samples and the biological activity of IL-1β showed that the expressed product could bind to its ligand and could be secreted into the cell culture supernatant.