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选择对多菌灵、乙霉威和苯酰菌胺具有不同敏感性的胶孢炭疽菌Colletotrichum gloeosporioides、辣椒疫霉菌Phytophthora capsici及恶疫霉菌P.cactorum,采用菌丝生长速率抑制法及氨基酸序列比对法分析了其β-微管蛋白氨基酸突变与敏感性的关系。结果表明,胶孢炭疽菌对苯酰菌胺、多菌灵和乙霉威的敏感性与β-微管蛋白198位或200位氨基酸突变有关:对多菌灵敏感、对苯酰菌胺和乙霉威不敏感的胶孢炭疽菌β-微管蛋白氨基酸198位为谷氨酸(E),200位为苯丙氨酸(F);对多菌灵已产生抗性而对苯酰菌胺和乙霉威不敏感的菌株,其β-微管蛋白氨基酸200位由苯丙氨酸(F)突变为了酪氨酸(Y);对多菌灵高抗、对苯酰菌胺和乙霉威敏感的菌株其β-微管蛋白氨基酸198位由谷氨酸(E)突变为了丙氨酸(A)。辣椒疫霉菌和恶疫霉菌对苯酰菌胺敏感,对多菌灵和乙霉威均不敏感。检测疫霉菌菌株β-微管蛋白未发现氨基酸突变,但发现其β-微管蛋白氨基酸在196～200位与胶孢炭疽菌差异较大,这可能是导致苯酰菌胺仅对疫霉菌有抑制效果的原因。
Colletotrichum gloeosporioides, Phytophthora capsici and P.cactorum were selected to have different sensitivities to carbendazim, diethylmyme and axenamid, and their mycelial growth rate and amino acid sequence ratio The analysis of the law of β-tubulin amino acid mutation and sensitivity of the relationship. The results showed that the sensitivity of Colletotrichum gloeosporioides to benzamidine, carbendazim and diethylbenzyl was related to mutation of amino acid at position 198 or 200 of β-tubulin: sensitive to carbendazim, Chelerythi insensitivity of Colletotrichum gloeosporioid β-tubulin amino acid 198 of glutamic acid (E), 200 for the phenylalanine (F); carbendazim has been resistant to benzoyl bacteria Amine and diethylbacterium insensitivity strains, the β-tubulin amino acid mutation from phenylalanine (F) to tyrosine (Y) at position 200; for carbendazim, The mold sensitive strain was mutated from glutamic acid (E) to alanine (A) at position 198 of the beta-tubulin amino acid. Phytophthora capsici and Phytophthora capsici are sensitive to axenamid and are insensitive to both carbendazim and diethylbenzyl. There was no amino acid mutation detected in the β-tubulin of Phytophthora infestans, but the β-tubulin amino acid was found to be significantly different from Colletotrichum gloeosporioides at the 196-200 position, Reasons to suppress the effect.