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目的 观察三氧化二砷(As_2O_3)对人鼻咽癌细胞株HNE1 LMP1 侵袭、转移的影响。方法 鼻咽癌细胞在含3μmol/L的As_2O_3培养基中培养 48 小时,然后在无含砷的培养基中继续培养 48 小时后,收集此时间点贴壁的细胞作为研究对象;用细胞 基质黏附实验、细胞运动实验和肿瘤细胞重组基底膜侵袭实验检测As_2O_3对HNE1 LMP1细胞黏附、运动及侵袭能力的影响;用激光共聚焦的方法检测EB病毒编码的潜伏膜蛋白1(LMP1)的表达情况。结果 肿瘤细胞 基质黏附实验结果显示,经 As_2O_3处理的HNE1 LMP1细胞,其黏附能力(平均吸光度为0.524±0.09)低于对照组细胞(平均吸光度为0.665±0.14),两者相比有差异(P<0.05)。运动实验和肿瘤细胞重组基底膜侵袭结果均显示,经 As_2O_3处理后,穿过游离的聚乙烯吡咯烷酮膜(PVP F)的肿瘤细胞数明显减少(P<0.01),同时 As_2O_3 能够下调LMP1的表达。结论 As_2O_3 具有抗人鼻咽癌细胞株转移的潜力,其机制可能与抑制 LMP1 的表达相关。
Objective To observe the effect of arsenic trioxide (As 2 O 3) on the invasion and metastasis of human nasopharyngeal carcinoma cell line HNE1 LMP1. Methods Nasopharyngeal carcinoma cells were cultured for 48 hours in 3μmol / L As_2O_3 medium and then cultured in arsenic-free medium for 48 hours. The adherent cells at this time point were collected as the research object. The effects of As_2O_3 on the adhesion, motility and invasion of HNE1 LMP1 cells were detected by experiments, cell motility test and tumor cell invasion assay. The expression of latent membrane protein 1 (LMP1) encoded by Epstein-Barr virus (EBV) was detected by laser scanning confocal microscope. Results Tumor cell matrix adhesion assay showed that the adhesion of HNE1 LMP1 cells treated with As_2O_3 (average absorbance 0.524 ± 0.09) was lower than that of the control cells (mean absorbance 0.665 ± 0.14) (P <0.05). The result of exercise test and tumor cell recombinant basement membrane invasion showed that the number of tumor cells decreased significantly (P <0.01) and the expression of LMP1 was down-regulated by As_2O_3 after free PVPF treatment. Conclusion As_2O_3 has the potential of anti-metastasis in human nasopharyngeal carcinoma cell lines, and its mechanism may be related to the inhibition of the expression of LMP1.