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报道3种化学合成的寡糖(β-1,3-乙酰氨基葡聚二糖,β-1,3-乙酰氨基葡聚三糖和Lewis~a三糖)诱导黄瓜对镰孢霉枯萎病(Fusariumoxysporumf.sp.cucumerium)的抗性.在黄瓜胚根期,β-1,3-乙酰氨基葡聚二糖和β-1,3-乙酰氨基葡聚三糖浸泡种子胚根后,表现抗病性,相对诱导抗性效果分别为21.9和22.3,β-1,3-乙酰氨基葡聚糖的最低有效诱导浓度为5μg·mL-1;而Lewis~a三糖处理不表现抗病性.在黄瓜幼苗期,用β-1,3-乙酰氨基葡聚二糖和β-1,3-乙酰氨基葡聚三糖叶面喷雾,均表现抗病性,相对诱导抗性效果分别是28.5和23.5,β-1,3-乙酰氨基葡聚糖的最低有效诱导浓度为10μg·mL-1;而Lewis~a三糖同样处理后不表现抗性.化学合成的几丁寡糖类似物(β-1,3-乙酰氨基葡聚二糖和β-1,3-乙酰氨基葡聚三糖)和Lewis~a三糖诱导黄瓜抗性的差异可能是由于糖残基、还原末端取代基及(或)其主链结构的差异.
It was reported that three kinds of chemical synthetic oligosaccharides (β-1,3-acetylglucosamine-disaccharide, β-1,3-acetylglucosaminoglycans and Lewis-a trisaccharide) Fusarium oxysporum f.sp.cucumerium. After cucumber radicle, β-1,3-acetylglucosamine-disaccharide and β-1,3-acetylglucosaminoglycans soaked seed radicle, the disease resistance The relative induction resistance was 21.9 and 22.3, respectively. The lowest effective concentration of β-1,3-aminodextran was 5μg · mL-1, while Lewis-a-trisaccharide did not show disease resistance. Cucumber seedling stage, with β-1,3-acetylglucosamine disaccharide and β-1,3-acetyl-glucosaminogalactan foliar spray, showed disease resistance, the relative induction resistance were 28.5 and 23.5 , the lowest effective induction concentration of β-1,3-acetylglucosamine was 10μg · mL-1, while Lewis-a trisaccharide did not show any resistance after the same treatment.Chemical synthesis of chitin oligosaccharide analogues (β- Differences in cucumber resistance induced by Lewis ~ a trisaccharide may be due to sugar residues, reduction of terminal substituents and (or ) The main structure of the difference.