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目的观察绿茶提取物EGCG对实验性自身免疫性脑脊髓炎(EAE)小鼠的治疗作用及相关机制。方法取C57BL/6小鼠,随机分为对照组、EAE模型组和EGCG治疗组,对各组小鼠进行神经功能评分,并对中枢神经系统炎性浸润情况进行观察;流式细胞技术检测Th1、Th17细胞在中枢神经系统中频率变化;免疫组化观察25 d脑组织IFN-γ、IL-17和TGF-β的表达情况;ELISA检测血清中IFN-γ、、IL-17、TGF-β、IL-10、IL-4的质量浓度。结果 EGCG治疗明显缓解EAE小鼠疾病症状和中枢神经系统炎症,抑制Th1与Th17细胞向中枢神经系统浸润。与EAE模型组相比,EGCG治疗组脑IFN-γ及IL-17表达上调,而TGF-β表达下调;外周血中可溶性上述细胞因子表达情况与脑一致。结论 EGCG可能通过影响中枢神经系统及外周血中IFN-γ、IL-17、TGF-β的表达,调控T细胞亚群的分化对EAE发挥保护作用。
Objective To observe the therapeutic effect and mechanism of EGCG, a green tea extract, on experimental autoimmune encephalomyelitis (EAE) mice. Methods C57BL / 6 mice were randomly divided into control group, EAE model group and EGCG treatment group. The neurological function scores of the mice in each group were observed and the infiltration of central nervous system was observed. Flow cytometry was used to detect Th1 , The frequency of Th17 cells in the central nervous system; Immunohistochemistry was used to observe the expression of IFN-γ, IL-17 and TGF-β in brain tissue at 25 days; The levels of IFN-γ, IL-17 and TGF- , IL-10, IL-4 mass concentration. Results EGCG treatment significantly alleviated the disease symptoms and central nervous system inflammation in EAE mice and inhibited the infiltration of Th1 and Th17 cells into the central nervous system. Compared with the EAE model group, the expression of IFN-γ and IL-17 in the EGCG treatment group was up-regulated, while the expression of TGF-β was down-regulated. The expression of soluble cytokines in the peripheral blood was consistent with the brain. Conclusion EGCG may protect EAE by regulating the expression of IFN-γ, IL-17 and TGF-β in the central nervous system and peripheral blood and regulating the differentiation of T cell subsets.