论文部分内容阅读
肿瘤相关抗原的存在可诱导宿主产生免疫应答,继而产生自身抗体.为揭示恶性乳腺肿瘤患者中这类免疫识别的范围及探讨肿瘤相关抗原的分子特性,著者用杂交瘤技术进行人-鼠种间杂交,成功地制备出乳癌人单抗,并用免疫组化方法进行了组织切片的细胞抗原检测.实验是取转移乳癌患者腋下淋巴结手术标本,无菌条件下制备成单细胞悬液.而后用常规PEG法与小鼠骨髓瘤细胞系M5进行融合.24小时后加入HAT培液,15～18天
The presence of tumor-associated antigens induces the host to produce an immune response, which in turn generates autoantibodies.To reveal the scope of such immune recognition in patients with malignant breast tumors and to explore the molecular properties of tumor-associated antigens, the authors used the hybridoma technique to conduct human- Hybrid, the successful preparation of breast cancer monoclonal antibody and immunohistochemical methods for the detection of cell antigen in tissue sections of the experiment is to take patients with metastatic breast cancer axillary lymph node specimens prepared under sterile conditions into a single cell suspension and then Conventional PEG method and mouse myeloma cell line M5 fusion 24 hours after adding HAT medium, 15 to 18 days