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目的了解云南省西部地区鼠类中巴尔通体(Bartonella species)的感染情况。方法2004年在景东、南华、盈江、龙陵等县捕捉活鼠,采集捕获鼠全血标本,用含5%兔心血的脑心浸液琼脂培养基分离巴尔通体,可疑标本用聚合酶链反应扩增枸橼酸合酶基因(gltA)的特异片段(379 bp) 加以证实。结果4个县共捕获鼠类397只,为1个属4个种,分属大足鼠、黄胸鼠、褐家鼠以及斑胸鼠。从397份标本中分离到巴尔通体54株,分离率为13.6%(54/397)。菌株分布于各调查点的鼠种中,大足鼠的分离率为22.0%(22/100)、黄胸鼠14.8%、(31/210)、褐家鼠为1.2%(1/87)、斑胸鼠阴性。结论云南省西部地区的鼠类中存在着较为广泛的巴尔通体的感染,还需要对相关疾病传播关系做进一步的研究。
Objective To understand the prevalence of Bartonella species in rodents in western Yunnan Province. Methods Live mice were harvested in Jingdong, Nanhua, Yingjiang, Longling and other counties in 2004. Blood samples were captured from whole blood of rats. Bartonella was isolated from brain heart infusion agar medium containing 5% The specific fragment of citrate synthase gene (gltA) was amplified by chain reaction (379 bp). Results A total of 397 rodents were captured in 4 counties, one species belonging to 4 genera, belonging to Dazu, Rattus flavipectus, Rattus norvegicus, and Zodiacal. 54 isolates of Bartonella were isolated from 397 specimens, the isolation rate was 13.6% (54/397). The strains were distributed in the rodents at each survey point. The isolation rate of dazu rats was 22.0% (22/100), the ratio of rostral tissues was 14.8% (31/210), that of Rattus norvegicus was 1.2% (1/87) Chest mice negative. Conclusion There is a more extensive Bartonella infection in rodents in the western region of Yunnan Province. Further study on the relationship between the transmission of the related diseases is needed.