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为了提高胶体金检测孔雀石绿方法的精密性、准确度、检测限等指标,并尽可能缩短样品处理时间,对样品用量、提取剂用量、离心时间、吹干温度、空气出量等检测条件进行优化,建立一种简便、快速检测水产品中孔雀石绿及其代谢物残留量的胶体金免疫层析方法。优化后,在满足检测限、假阳率和假阴率不变的条件下,样品处理过程更加简单,省去了传统方法需要过柱的步骤;单个样本处理,除去空气吹干的时间,只要最少7min即可完成样品的前处理,大大低于市场上样品前处理的时间;出带速度快,检测时间短,单个样本的检测时间在10~15min;对组织(鱼)中孔雀石绿的检出限可提高到2μg/kg;减少样品处理过程中耗材和试剂的用量,节约检测成本。该方法节省了大量的人力和物力,适用于日常的大批量样品检测,为胶体金免疫层析法检测孔雀石绿及其代谢物,提供了一种新的样品处理方法。
In order to improve the precision, accuracy and detection limit of the malachite green method for colloidal gold detection, and to shorten the sample processing time as much as possible, the detection conditions of sample amount, extractant dosage, centrifugation time, drying temperature and air output Optimization, to establish a simple and rapid detection of aquatic malachite green and its metabolite residues of colloidal gold immunochromatography. After the optimization, under the condition of meeting the detection limit, false positive rate and false negative rate, the sample processing process is simpler, eliminating the need for the traditional method to pass the column. Single sample processing removes the air drying time as long as At least 7min to complete the pretreatment of the sample, which is much lower than the sample pretreatment time on the market; the speed of the belt is fast, the detection time is short, the detection time of a single sample is 10-15min; the malachite green The detection limit can be increased to 2μg / kg; reduce the amount of consumables and reagents used in the sample processing, saving detection costs. The method saves a lot of manpower and material resources, and is suitable for daily sample testing in large quantities. The method provides a new sample processing method for detecting the malachite green and the metabolites by a colloidal gold immunochromatography method.