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Proline-rich protein 11(PRR11)是一个与细胞周期调控和肿瘤发生发展均密切相关的新基因。前期研究表明,PRR11敲减后导致肿瘤细胞周期S期阻滞。该研究利用流式细胞检测和免疫荧光方法在人肺癌细胞系H1299中分析siRNA介导的PRR11表达沉默对细胞周期产生的影响,通过添加dNTPs分析核糖核苷酸还原酶表达下降在PRR11敲降导致的S期阻滞中的作用。Brdu标记结果表明,PRR11敲减阻滞细胞周期在S期,并且核糖核苷酸还原酶表达下降导致细胞周期阻滞。使用分裂期标记蛋白pHH3分析结果表明,PRR11下调表达同时也导致细胞在G2期产生阻滞,从而抑制细胞进入有丝分裂。研究结果表明,在细胞周期过程中抑制PRR11的表达会阻滞DNA合成和有丝分裂进行,推测PRR11异常表达可能导致细胞周期紊乱,从而促进肿瘤的发生发展。
Proline-rich protein 11 (PRR11) is a novel gene closely related to the regulation of cell cycle and the development of tumor. Preliminary studies have shown that knockdown of PRR11 results in arrest of S phase of tumor cell cycle. This study used flow cytometry and immunofluorescence to analyze the effect of siRNA-mediated silencing of PRR11 on cell cycle in a human lung cancer cell line H1299. The knockdown of PRR11 resulted from the decrease of ribonucleotide reductase expression by adding dNTPs S phase arrest in the role. Brdu labeling results showed that PRR11 knockdown blocked cell cycle in S phase, and decreased expression of ribonucleotide reductase led to cell cycle arrest. Using split-phase marker protein pHH3 analysis results show that down-regulation of PRR11 also leads to cells in G2 arrest, thus inhibiting the cells into mitosis. The results showed that inhibition of PRR11 expression during cell cycle blocked DNA synthesis and mitosis, suggesting that abnormal expression of PRR11 may lead to cell cycle disorders, thereby promoting tumor development.