论文部分内容阅读
以葡萄品种Melissa、Sweet Scarlet、巨玫瑰、黑玫瑰组培苗为试验材料,在继代培养基中加入不同浓度的白砂糖、甘露醇提高渗透压或分别附加不同浓度的多效唑(PP333)、矮壮素(CCC)、脱落酸(ABA)等生长调节剂,研究其对葡萄组培苗离体保存延缓生长的影响,以探寻适宜葡萄离体种质保存期间延长继代间隔的方法。结果表明:培养基中附加20~30 g/L甘露醇或附加2~3mg/LPP333能有效抑制Melissa、巨玫瑰葡萄组培苗的生长,存活率高,可用于该葡萄品种种质离体保存。培养基中附加25~85g/L的白砂糖对Melissa、巨玫瑰葡萄组培苗未表现抑制生长作用;低于300mg/L的CCC对Melissa、Sweet Scarlet葡萄组培苗抑制生长作用不明显,高于400 mg/LCCC引起组培苗玻璃化。附加3 mg/L以上ABA对巨玫瑰、黑玫瑰葡萄组培苗抑制生长作用不明显,且引起组培苗死亡。CCC和ABA不适宜上述供试葡萄组培苗的缓慢生长法保存。
With the grape varieties Melissa, Sweet Scarlet, giant rose and black rose tissue culture seedlings as test materials, different concentrations of sugar and mannitol were added to the subculture medium to increase the osmotic pressure or add different concentrations of PP333, (CCC) and abscisic acid (ABA) were used to study the effects of CCC and delayed abscisic acid (ABA) on the delayed growth of grape seedlings in vitro. The results showed that adding 20-30 g / L of mannitol or adding 2-3 mg / L of LP333 could effectively inhibit the growth of Melissa and giant rose grape seedlings and had high survival rate, which could be used for in vitro preservation of the grape variety . The addition of 25 ~ 85g / L sugar to medium had no effect on the growth inhibition of Melissa and Jujubegrass grapevine. The CCC less than 300mg / L had no obvious inhibitory effect on the growth of Melissa and Sweet Scarlet Vitrification of tissue culture seedlings was induced at 400 mg / L CCC. The addition of 3 mg / L ABA on giant rose, black rose grape seedling growth inhibition is not obvious, and caused tissue culture seedlings death. CCC and ABA are unsuitable for the above slow-growing method of tissue culture seedlings for grapes.