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模式植物miRNA398可以抑制靶基因Cu/Zn-SOD的表达。为揭示烟草miRNA398的生物学功能,采用荧光定量PCR技术分析了烟草品种CB-1和B37在干旱胁迫条件下miRNA398和Cu/Zn-SOD基因的表达量,并预测了烟草miRNA398结合Cu/Zn-SOD基因的位点。结果表明:烟草miRNA398与Cu/Zn-SOD基因5非翻译区的21个碱基序列互补,从而抑制该基因的表达;在干旱胁迫条件下,CB-1的miRNA398表达水平上升,B37的miRNA398表达水平下降,Cu/Zn-SOD基因表现出与miRNA398相反的表达趋势,说明抗旱能力存在差异的两个品种中miRNA398均可抑制Cu/Zn-SOD基因表达,并且对干旱胁迫响应具有两种不同的方式。
Model plant miRNA398 can inhibit the target gene Cu / Zn-SOD expression. To reveal the biological function of tobacco miRNA398, the expression levels of miRNA398 and Cu / Zn-SOD genes in tobacco varieties CB-1 and B37 under drought stress were analyzed by fluorescence quantitative PCR, SOD gene locus. The results showed that the miRNA398 of tobacco was complementary to the 21 base sequence in the untranslated region of Cu / Zn-SOD gene, thereby inhibiting the expression of miRNA398. Under drought stress, the expression of miRNA398 in CB-1 increased and that of miRNA398 in B37 The level of Cu / Zn-SOD gene showed the opposite trend to that of miRNA398, indicating that miRNA398 inhibited the Cu / Zn-SOD gene expression in two cultivars with different drought tolerance ability and had two different responses to drought stress the way.