谷胱甘肽过氧化物酶(GSH-Px)是体内一种重要的抗脂质过氧化物酶,近年来,免疫酶技术在研究GSH-Px细胞内分布、代谢等方面得到广泛应用。这种技术需要重复性好,效果优良的标记法和高效稳定的酶标抗体。传统的辣根过氧化物酶(HRP)标记抗体的过碘酸钠法(Nakane法),酶活性显著下降和IgG-HRP大分子聚合物的形成,影响了GSH-Px抗原抗体反应的敏感性,为克服这一缺陷,木文采用一种新的HRP标记IgG过碘酸钠法,将标记物用于GSH-Px的酶联免疫吸附测定(ELISA)法、免疫组化及免疫电镜研究,取得满意结果。 Glutathione peroxidase (GSH-Px) is an important anti-lipid peroxidase in the body. In recent years, it has been widely used in studying the distribution and metabolism of GSH-Px cells. This technique requires good reproducibility, good labeling and efficient and stable ELISA. Conventional HRP labeled antibodies by sodium periodate (Nakane method), a significant decline in enzyme activity and IgG-HRP macromolecular polymer formation, affecting the sensitivity of GSH-Px antigen antibody response In order to overcome this shortcoming, we used a new HRP-labeled IgG sodium periodate method to detect GSH-Px by enzyme-linked immunosorbent assay (ELISA), immunohistochemistry and immunoelectron microscopy. Achieve satisfactory results.