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目的了解不同种类透析膜对外周血单个核细胞(PBMC)产生白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNFα)、IL-6的影响。方法分离培养PBMC,用放射免疫(RIA)法测定培养上清液中IL-1β、TNFα、IL-6。结果用铜仿(CU)膜透析,透前PBMC产生的IL-1β、TNFα、IL-6高于对照组;其次是聚甲基丙烯酸甲脂膜(PMMA)膜和HE膜。透析过程中PBMC产生的细胞因子都明显减少,与透前相比有显著性差异,其中以用CU膜透析下降最为明显。透析结束时,接近甚至超过透析前水平。HE膜用过氧乙酸或福尔马林消毒第四次复用与首次使用相比,对PBMC产生细胞因子的影响无明显差异。用简单直线回归的方法分析发现透前、透析15分钟、240分钟时PBMC产生的IL-1β、TNFα、IL-6之间呈正相关。结论 (1)不同种类透析膜对透析过程中PBMC在脂多糖(LPS)刺激下产生IL-1β、TNFα、IL-6的影响不同;CU>PMMA、HE>PSN;(2)在透析过程中,同一时间获得的PBMC在LPS刺激下产生的IL-1β、TNFα、IL-6之间呈正相关;(3)用过氧乙酸或福尔马林消毒复用对PBMC产生细胞因子无明显影响。
Objective To investigate the effects of different types of dialysis membranes on interleukin-1β (IL-1β), tumor necrosis factor-α (TNFα) and interleukin-6 (IL-6) production in peripheral blood mononuclear cells (PBMC) Methods PBMCs were isolated and cultured, and IL-1β, TNFα and IL-6 in culture supernatant were determined by radioimmunoassay (RIA). Results CU membrane was used for dialysis. The levels of IL-1β, TNFα and IL-6 produced by PBMC were higher than those of the control group, followed by PMMA membrane and HE membrane. During the process of dialysis, the cytokines produced by PBMC were significantly decreased compared with those before the dialysis, of which the most obvious decrease was the dialysis with CU membrane. At the end of dialysis, it approaches or exceeds pre-dialysis levels. The fourth reuse of HE membrane with peracetic acid or formalin disinfection had no significant effect on the production of cytokines by PBMC compared with the first use. Analysis by simple linear regression revealed that there was a positive correlation between IL-1β, TNFα and IL-6 produced by PBMC at 15 minutes and 240 minutes after dialysis. Conclusions (1) Different kinds of dialysis membranes have different effects on the production of IL-1β, TNFα and IL-6 by PBMC during LPS stimulation during dialysis; CU> PMMA and HE> PSN; (2) , While the level of IL-1β, TNFα and IL-6 produced by LPS stimulated by PBMCs at the same time was positively correlated. (3) There was no significant effect on the production of cytokines by PBMCs with peracetic acid or formalin.