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目的:观察中医不同治法方药对大鼠心肌缺血再灌注损伤(I/R)核因子-кB(NF-кB)信号转导通路的影响。方法:将60只SD大鼠随机分为5组:假手术组、模型组、祛痰宽胸组、活血化瘀组及痰瘀同治组。可逆性冠脉左前降支结扎缺血30 min再灌注2 h复制I/R模型,应用Western blotting及RT-PCR法检测各组大鼠心肌核因子IкB诱导激酶(NIK)、IкB激酶β(IKKβ)、核转录因子抑制蛋白(IкBα)及NF-кBp65 mRNA的表达。结果:与模型组比较,各治疗组均能降低心肌NIK,IKKβ,NF-кBp65 mRNA水平并上调IкBα蛋白表达(P<0.01)。结论:祛痰宽胸法、活血化瘀法及痰瘀同治法均对I/R大鼠有保护作用,其主要作用机制可能是通过抑制NIK,IKKβ蛋白表达,减少IкBα蛋白的磷酸化降解而增加其蛋白水平,从而抑制NF-кB的过度活化,发挥其对缺血再灌注损伤心肌的保护作用。
Objective: To observe the effect of different traditional Chinese medicine prescriptions on nuclear factor-kappa B (NF-κB) signal transduction pathway in rats with myocardial ischemia-reperfusion injury (I / R). Methods: Sixty Sprague-Dawley rats were randomly divided into 5 groups: sham-operation group, model group, expectorant wide-thorax group, Huoxuehuayu group and Tongxu-Tongzhi group. Reversible left anterior descending coronary artery was ligated for 30 min and then reperfused for 2 h for I / R model. Western blotting and RT-PCR were used to detect the expression of nuclear factor IкB-inducible kinase (NIK), IκB kinase β (IKKβ ), Nuclear transcription factor inhibitor protein (IкBα) and NF-кBp65 mRNA expression. Results: Compared with the model group, all the treatment groups could reduce the levels of NIK, IKKβ and NF-κBp65 mRNA and up-regulate the expression of IκBα protein (P <0.01). Conclusion: Expectorant and broad-minding methods, promoting blood circulation and removing blood stasis and treating phlegm and blood stasis have the protective effect on I / R rats. The main mechanism may be through inhibiting the expression of NIK and IKKβ proteins and decreasing the phosphorylation and degradation of IкBα protein Increase its protein level, thereby inhibiting NF-кB over-activation, exert its protective effect on myocardial ischemia-reperfusion injury.