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基于纳米金对荧光素的内滤光效应,建立了一种新型高灵敏、高选择性测定半胱氨酸的分析方法。将具有较大摩尔吸光系数的纳米金选为吸收体,荧光素的荧光光谱与纳米金的吸收光谱能很好地重叠,作为荧光发光体。在选定的实验条件下,荧光素荧光强度的增加值与半胱氨酸的浓度在5.0×10-8～1.0×10-6mol/L范围内呈良好的线性关系,检测限为1×10-8mol/L。
Based on the internal filtering effect of gold nanoparticles on fluorescein, a new sensitive and selective method for the determination of cysteine was established. The nanometer gold with larger molar extinction coefficient is selected as the absorber, and the fluorescence spectrum of fluorescein and the absorption spectrum of gold nanogold can be well overlapped as the fluorescent luminescent material. Under the selected experimental conditions, the increase of fluorescence intensity of fluorescein showed a good linear relationship with the concentration of cysteine in the range of 5.0 × 10-8 ~ 1.0 × 10-6mol / L with the detection limit of 1 × 10 -8mol / L.