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为检测二羟基乙二肟（ＤＨＧ）对细胞的恶性转化能力，判断其体外致癌性，本研究用ＤＨＧ对ＢＡＬＢ／ｃ－３Ｔ３细胞进行转化试验。结果表明：ＤＨＧ能诱导ＢＡＬＢ／ｃ－３Ｔ３细胞发生转化，转化细胞可使刀豆球蛋白（ＣｏｎＡ）凝集并可在软琼脂上生长，将其接种于ＢＡＬＢ／ｃ裸小鼠皮下能诱发出纤维肉瘤。以上结果充分说明ＤＨＧ诱导的ＢＡＬＢ／ｃ－３Ｔ３细胞的转化为恶性转化，预示该化学物对人类具有潜在致癌危险性
In order to detect the malignant transformation ability of dihydroxy ethanedipine (DHG) cells and determine its in vitro carcinogenicity, this study used DHG to perform transformation experiments on BALB/c-3T3 cells. The results showed that: DHG can induce the transformation of BALB/c-3T3 cells, transformed cells can concanine (ConA) agglutination and can grow on soft agar, it can be inoculated in subcutaneous BALB / c mice can induce fiber sarcoma. The above results fully demonstrate that the conversion of BALB/c-3T3 cells induced by DHG to malignant transformation indicates that the chemical has potential carcinogenic potential to humans.