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目的建立发育期戊四氮(PTZ)点燃癫大鼠模型,观察银杏叶提取物(EGb)对发育期点燃癫大鼠的干预作用,并探讨其干预机制,为拓展EGb的临床应用提供实验依据。方法 40只健康21日龄SD大鼠随机分成5组:正常对照组、PTZ+50 mL.L-1乙醇7 d组、PTZ+50 mL.L-1乙醇14 d组、PTZ+EGb 7 d组、PTZ+EGb 14 d组,每组8只。观察点燃及治疗过程中各组大鼠的发作情况,以Y型-电迷宫及Morris水迷宫评价各组大鼠的认知功能,并以病理方法评价海马神经细胞凋亡的情况。采用SPSS 16.0软件进行统计学分析。结果 EGb治疗后点燃癫大鼠癫发作的次数减少,Y型-电迷宫实验中达标次数明显减少,Morris水迷宫实验中逃避潜伏期明显缩短,穿越平台次数明显增多,原位末端标记(TUNEL)显示实验中海马CA3区神经细胞凋亡明显减少,但是EGb治疗14 d组与7 d组比较差异无统计学意义。结论 EGb治疗7 d和14 d均可改善发育期点燃癫大鼠的认知功能,但是并未显示出14 d疗程比7 d疗程有明显的疗效优势,此药理作用可能与减少或阻断海马神经细胞凋亡有关。
OBJECTIVE: To establish a rat model of PTZ-induced epileptic epilepsy and to observe the intervention effect of Ginkgo biloba extract (EGb) on the epileptic seizure rats during development and to explore its mechanism of intervention in order to expand the clinical application of EGb Experimental basis. Methods Forty healthy 21-day-old SD rats were randomly divided into five groups: normal control group, PTZ + 50 mL.L-1 ethanol for 7 d, PTZ + 50 mL.L-1 ethanol for 14 d, PTZ + EGb for 7 d Group, PTZ + EGb 14 d group, 8 rats in each group. The incidence of the rats in each group during the process of ignition and treatment was observed. The cognitive function of rats in each group was evaluated by Y-electric maze and Morris water maze. The apoptosis of hippocampal neurons was evaluated by pathology. SPSS 16.0 software was used for statistical analysis. Results After EGb treatment, the number of epileptic seizures in the epileptic rats was reduced, the number of the standard epilepsy was significantly reduced in the Y-type electric maze test, the escape latency was significantly shortened and the number of traversing platforms was significantly increased in the Morris water maze test. The TUNEL ) Showed that in the hippocampal CA3 region, the apoptosis of neurons decreased significantly, but there was no significant difference between the 14th and the 7th day after EGb treatment. Conclusion EGb treatment can improve the cognitive function of rats with epileptic seizure at developmental stage on the 7th and 14th day after EGb treatment. However, EGb treatment did not show obvious therapeutic advantage over the 7th day after 14 days treatment, which may be related to the reduction or interruption Hippocampal nerve cell apoptosis related.