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目的:研究毛蚶糖胺聚糖(SSG)对高脂血症小鼠的降血脂作用以及对动物细胞内胆固醇生物合成的影响。方法:试验持续4周,用试剂盒测定血清中TC、TG、LDL-C、HDL-C的含量;取小鼠肝脏,石蜡切片,光镜观察小鼠肝脏细胞;通过体外培养中国仓鼠卵细胞(CHO),用两性霉素B-细胞模型法观察SSG对细胞内胆固醇生物合成的抑制作用,用酶联免疫法检测CHO细胞的存活率。结果:SSG中、高剂量能显著降低高脂血症小鼠血清中TC、TG、LDL-C含量和AI(LDL-C/HDL-C)值(P<0.05),使高脂血症小鼠的脂肪肝有明显的减轻;毛蚶SSG2-3-2、SSG2-3-1有保护CHO细胞免受两性霉素B损伤作用(P<0.01)。结论:毛蚶糖胺聚糖对实验性高血脂小鼠具有显著的降血脂作用和预防脂肪肝作用,其作用机制与细胞内胆固醇的生物合成抑制有关。
Objective: To study the hypoglycemic effect of Sarcogon glycosaminoglycan (SSG) on hyperlipidemia mice and its effect on cholesterol biosynthesis in animal cells. Methods: The serum concentration of TC, TG, LDL-C and HDL-C were determined by kit. The liver and paraffin sections of mice were observed by light microscope and the liver cells of mice were observed by light microscope. CHO). The inhibitory effect of SSG on cholesterol biosynthesis was observed by amphotericin B-cell model, and the survival rate of CHO cells was detected by enzyme-linked immunosorbent assay. Results: The levels of TC, TG, LDL-C and AI (LDL-C / HDL-C) in the serum of the mice with hyperlipidemia were significantly lower (P <0.05) The fatty liver of rats was significantly alleviated. The catgut-毛 SSG2-3-2 and SSG2-3-1 had the protective effect against amphotericin B (P <0.01). CONCLUSION: Gansu glycosaminoglycan has significant hypolipidemic effect and prevention of fatty liver in experimental hyperlipidemia mice. The mechanism of action is related to the inhibition of intracellular cholesterol biosynthesis.