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目的:采用UPLC-ESI-MS-MS方法比较生晒参和紫红参中人参皂苷类成分的种类及含量,阐明紫红参特异成分。方法:Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm)色谱柱,流动相A(0.1%甲酸)-B(乙腈)梯度洗脱,柱温30℃;质谱条件:电喷雾离子化源(ESI),负离子检测模式。结果:与生晒参相比,紫红参中人参皂苷的种类和数量发生了改变,检测出含量较高的人参皂苷为20(S)-人参皂苷Rg3、20(R)-人参皂苷Rg3、20(R)-人参皂苷Rh1、20(R)-人参皂苷Rg2,Rk1,Rg5,Rd,Ro、姜状三七苷R1等。结论:人参加工成紫红参后皂苷类成分发生较大的变化,产生了大量稀有皂苷,人参加工可能产生紫红参的特异成分。
OBJECTIVE: To compare the types and contents of ginsenosides in Radix Houttuynia and Radix Ginseng by UPLC-ESI-MS-MS and clarify the specific components of Radix Ginseng. Methods: Waters ACQUITY UPLC BEH C18 (2.1 mm × 50 mm, 1.7 μm) column was used. The mobile phase A (0.1% formic acid) -B (acetonitrile) (ESI), negative ion detection mode. Results: Compared with raw ginseng, the type and amount of ginsenosides in purple ginseng changed. The higher ginsenosides detected were 20 (S) - ginsenoside Rg3, 20 (R) - ginsenoside Rg3, (R) -ginsenoside Rh1,20 (R) -ginsenoside Rg2, Rk1, Rg5, Rd, Ro, Ginger pallucoside R1 and the like. Conclusion: Ginseng processed into purple red ginseng saponins after a larger change, resulting in a large number of rare saponins, ginseng processing may produce purple ginseng specific components.