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目的:建立测定益智挥发油中p-聚伞花烃含量的方法。方法:采用气相色谱法,HP-5弹性石英毛细管色谱柱(30m×0.32mm×0.25μm),载气为N2,流速1mL·min-1,进样口温度200℃,检测器温度280℃,程序升温,外标法计算含量。结果:p-聚伞花烃在0.43～4.30mg·mL-1范围内线性关系良好(r=0.9999);平均加样回收率为97.1%,RSD(n=6)为0.6%。结论:本方法简单、灵敏,重复性好,可用于益智挥发油中p-聚伞花烃的含量测定。
Objective: To establish a method for the determination of p-cymene in Volatile Oil from Yam. Methods: Gas chromatography was used on a HP-5 elastic silica capillary column (30m × 0.32mm × 0.25μm). The carrier gas was N2, the flow rate was 1mL · min-1, the inlet temperature was 200 ℃, the detector temperature was 280 ℃, Program warming, external standard calculation of content. Results: The linear range of p-cymene was 0.43 ~ 4.30 mg · mL-1 (r = 0.9999). The average recovery was 97.1% and RSD (n = 6) was 0.6%. Conclusion: The method is simple, sensitive and reproducible. It can be used to determine the content of p-cymene in the volatile oil.