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作者从曼氏血吸虫的成虫和虫卵中分离出信使核糖核酸(mRNA)并在体外转译,经鉴定体外转译产物为多肽,存在于血吸虫童虫的表面。按Smithers报告的方法收集曼氏血吸虫成虫,虫卵按Doenhoff方法制备,经生理盐水洗后液氮冰冻。用机械方法由尾蚴转变而得童虫,培养于NGTG-135培养基中,置37℃C含CO_2气体中孵育18小时。用此童虫加弗氏完全佐剂重复皮下注射免疫兔获得兔抗童虫血清。人血清采自曼氏血吸虫病患者。mRNA的分离及体外转译,系将液氮冰
The authors isolated messenger RNA (mRNA) from adult and insect eggs of Schistosoma mansoni and translated it in vitro. The in vitro translatable product was identified as polypeptide, which is present on the surface of the schistosomula. The adult Schistosoma mansoni was collected by the method reported by Smithers. The eggs were prepared according to the Doenhoff method. After washing with physiological saline, the liquid nitrogen was frozen. Schistosoma japonicum was transformed by mechanical means to Schistosoma japonicum, cultured in NGTG-135 medium and incubated in CO 2 -containing gas at 37 ° C for 18 hours. Rabbit anti-sperm serum was obtained by repeated subcutaneous injections of immunized rabbits with this schistosomiasis plus Freund’s complete adjuvant. Human serum collected from patients with Schistosoma mansoni. Isolation of mRNA and in vitro translation, the Department of liquid nitrogen ice