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目的建立测定益心胶囊中丹参酮ⅡA含量的方法。方法采用HPLC法测定益心胶囊中丹参酮ⅡA的含量。色谱柱为Agilent C18,流动相为甲醇-水(80∶20),流速为1.2 ml·min-1,检测波长为270 nm,柱温为30℃,进样量为10μl。结果益心胶囊中丹参酮ⅡA的进样量在1.760~44.00μg范围内与峰面积呈良好的线性关系(r=0.9995),平均回收率为99.47%,RSD为0.49%(n=9)。结论该方法操作简便、快捷、准确、可靠,可用于益心胶囊中丹参酮ⅡA含量的质量控制。
Objective To establish a method for the determination of tanshinone Ⅱ A in Yixin Capsule. Methods The content of tanshinone Ⅱ A in Yixin Capsules was determined by HPLC. The column was Agilent C18 with a mobile phase of methanol-water (80:20) at a flow rate of 1.2 ml · min-1 at a detection wavelength of 270 nm with a column temperature of 30 ° C and an injection volume of 10 μl. Results The injection of tanshinone ⅡA in Yixin capsule showed a good linear relationship with the peak area in the range of 1.760 ~ 44.00 μg (r = 0.9995). The average recovery was 99.47% and the RSD was 0.49% (n = 9). Conclusion The method is simple, rapid, accurate and reliable and can be used for the quality control of Tanshinone Ⅱ A in Yixin Capsules.