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目的:建立测定四氢异喹啉(Sal)和五羟色胺(5-HT)的电分析化学新方法。方法:首先通过电化学方法制备了铂纳米/碳纳米管复合材料(Pt NPs/MWCNTs)修饰的玻碳电极,并通过扫描电镜研究了复合材料形貌,同时也研究了Sal和5-HT在Pt NPs/MWCNTs修饰电极上的电化学行为。结果:Sal和5-HT的电化学氧化信号有着明显的分离,对于Sal,峰电流与4.8×10-7~5.2×10-5mol·L-1呈线性变化(r=0.9995),检测限为1.6×10-7mol·L-1(S/N=3);而对于5-HT,峰电流与5-HT在2.7×10-7~3.07×10-5mol·L-1范围内呈线性增加(r=0.9993),检测限为9.0×10-8mol·L-1(S/N=3)。结论:本方法可以应用于血样中Sal和5-HT的同时灵敏检测,结果令人满意。
Objective: To establish a new method for the determination of tetrahydroisoquinoline (Sal) and serotonin (5-HT). Methods: The glassy carbon electrode modified by Pt NPs / MWCNTs was prepared by electrochemical method. The morphology of the composite was investigated by scanning electron microscopy. The effects of Sal and 5-HT on Electrochemical Behavior of Pt NPs / MWCNTs Modified Electrode. Results: The electrochemical oxidation of Sal and 5-HT showed obvious separation. For Sal, the peak current linearly changed from 4.8 × 10-7 to 5.2 × 10-5 mol·L-1 (r = 0.9995), the detection limit was 1.6 × 10-7mol·L-1 (S / N = 3). For 5-HT, the peak current increased linearly with 5-HT in the range of 2.7 × 10-7 ~ 3.07 × 10-5mol·L-1 (r = 0.9993). The detection limit was 9.0 × 10-8mol·L-1 (S / N = 3). Conclusion: This method can be applied to the simultaneous and sensitive detection of Sal and 5-HT in blood samples with satisfactory results.