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本文研究新型毒品5F-AMB在体外肝微粒体模型中的代谢物。在体外人肝微粒体孵育模型中加入5F-AMB以模拟在人体的代谢过程,并利用超高效液相色谱高分辨质谱联用技术(UPLC-HR-MS)分析代谢发生位点及代谢途径。结果显示,5F-AMB在人肝微粒体模型中共产生9种代谢物,其主要代谢途径为酯键水解、酯键水解合并脱氟氧化、酯键水解合并戊烷基链羟化和酯键水解合并吲唑环羟化。以上结果表明该方法简便快捷,推荐5F-AMB的酯键水解、酯键水解合并脱氟氧化、酯键水解合并戊烷基链羟化和酯键水解合并吲唑环羟化代谢物为合适的潜在尿液标志物。
This article studies the metabolites of the novel drug 5F-AMB in an in vitro liver microsomal model. 5F-AMB was added to in vitro human liver microsomal incubation model to simulate the metabolic process in the human body, and the metabolic loci and metabolic pathways were analyzed by UPLC-HR-MS. The results showed that 5F-AMB produced 9 metabolites in human liver microsome model. The main metabolic pathways of 5F-AMB were ester hydrolysis, ester hydrolysis and defluorination, ester hydrolysis and pentyl chain hydroxylation and ester hydrolysis Merging indazole ring hydroxylation. The above results show that the method is simple and quick. It is recommended that 5F-AMB ester hydrolysis, ester hydrolysis and defluorination oxidation, ester hydrolysis and pentaalkyl chain hydroxylation and ester hydrolysis and indazole hydroxylation metabolites are suitable Potential urine markers.