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目的:制备包裹肿瘤特异性抗原MAGE3/HSP70-FITC的纳米脂质体,研究其特性及树突状细胞(DC)对其摄取的情况。方法:采用薄膜分散法,制备包裹MAGE3/HSP70-FITC的纳米脂质体(简称NEMH-FITC),观察其形态并测量其粒径,凝胶层析法检测纳米脂质体的包裹率和载药量,并检测其稳定性。将人外周血DC与NEMH-FITC共育后,用激光共聚焦显微镜观察DC摄取NEMH-FITC的情况。结果:成功地制备出平均粒径为95nm左右的纳米脂质体,其包裹率为37%,载药量为0.037g/L,于4℃放置6个月后性质稳定。激光共聚焦显微镜下观察到NEMH-FITC可被DC摄取。结论:纳米脂质体具有良好的包裹率和载药量,可有效地增强抗原递呈细胞对所载药物的摄取,可作为肿瘤抗原疫苗的新型载体。
OBJECTIVE: To prepare nano-liposomes encapsulating the tumor-specific antigen MAGE3 / HSP70-FITC and study its characteristics and the uptake by dendritic cells (DCs). Methods: Nano-liposomes coated with MAGE3 / HSP70-FITC (NEMH-FITC) were prepared by membrane dispersion method. Morphology was observed and the particle size was measured. The encapsulation efficiency of nano-liposomes Dose, and test its stability. After incubating human peripheral blood DC with NEMH-FITC, the situation of DC uptake of NEMH-FITC was observed by laser confocal microscopy. Results: The nanosized liposomes with an average diameter of about 95nm were successfully prepared. The encapsulation efficiency was 37% and the drug loading was 0.037g / L. After being placed at 4 ℃ for 6 months, the liposomes were stable in nature. Laser confocal microscopy showed that NEMH-FITC was uptake by DCs. Conclusion: Nano-liposomes have good encapsulation efficiency and loading capacity, which can effectively enhance the uptake of drugs by antigen-presenting cells and can be used as a new carrier of tumor antigen vaccine.