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为探讨人早期胎盘组织产生的促肾上腺皮质激素释放激素（CRH）的生物学作用及其绒毛膜促性腺激素（hCG）合成和释放的调控机制，在人早期胎盘组织体外孵育模型上研究了CRH及其拮抗剂（α－HelicalCRF9-41）对hCG基础分泌和由GnRH刺激的hCG分泌的影响。结果表明，10-10～10-7mol／L的外源性CRH可明显抑制hCG的基础释放（P＜0.05），而以10-7mol／L作用最显著（P＜0．001）；加入10-7mol／L的CRH4小时后，hCG释放量开始有所下降，但尚无统计学差异，到加药后8小时，CRH组已明显低于相应的对照组（P＜0．05），以加药后16小时抑制作用最明显（P＜0．001），到24小时抑制作用趋于减弱，但与同期对照相比仍有显著差异（P＜0．05）；10-7mol／L和10-5mol／L的α-HelicalCRF9-41可明显刺激hCG的分泌（P＜0.001）；2×10-7mol／L和2×10-6mol／L的α－HelicalCRF9-41可完全逆转CRH对hCG释放的抑制作用；2×10-9～2×10-7mol／L的CRH对由GnRH诱导的hCG释放有明显的抑制作用。这些结果提示，由胎盘的合体滋养层产生的CRH，通过受体介导机制，不仅对hCG基础分泌，且对由GnRH诱导的hCG分泌都有明显的抑制作用，且这种作用具有特定的时间依赖性和剂量依赖性．CRH可能是参与hCG分泌调控的生理性负调节团子之一.
To investigate the biological role of corticotropin-releasing hormone (CRH) produced by human early placenta tissue and the regulatory mechanism of its synthesis and release of human chorionic gonadotropin (hCG), we studied the effects of CRH And its antagonist (α-Helical CRF9-41) on hCG basal secretion and GnRH-stimulated hCG secretion. The results showed that exogenous CRH (10-10 ~ 10-7mol / L) significantly inhibited the basal release of hCG (P <0.05) and the effect of 10-7mol / L (P <0.001) After 7 hours of CRH treatment, the release of hCG began to decrease, but there was no statistical difference. After 8 hours of dosing, the CRH group was significantly lower than the corresponding control group (P <0.05) The inhibitory effect was most obvious at 16 hours after dosing (P <0.001), and the inhibition effect tended to decrease at 24 hours, but still significantly different from the control at the same period (P <0.05) Α-Helical CRF9-41 (10-5 mol / L) significantly stimulated the secretion of hCG (P <0.001); 2 × 10-7 mol / L and 2 × 10-6 mol / L α-Helical CRF9-41 completely reversed the effect of CRH on hCG Release of 2 × 10-9 ~ 2 × 10-7mol / L of CRH on GnRH-induced hCG release significantly inhibited. These results suggest that CRH, produced by the syncytiotrophoblast of the placenta, not only basal hCG secretion but also GnRH-induced hCG secretion through a receptor-mediated mechanism, and this effect has a specific time Dependency and dose dependence. CRH may be involved in the regulation of hCG secretion of one of the physiological negative regiment.