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【目的】研究新疆陆地棉叶绿素含量和光合速率的QTL定位,为陆地棉高光效分子标记辅助选择育种提供理论指导。【方法】以高光效的陆地棉品系ZX1789和低光效陆地棉品系E563号组配衍生的(ZX1789×E563)F2和F2∶3家系为材料,应用复合区间作图法,对F2∶3家系的叶绿素含量、光合速率进行QTL定位。【结果】构建了包括9个连锁群、标记间平均距离14.7 cM、全长为587.3 cM的遗传连锁图谱,约占棉花基因组的11.6%。检测到1个与叶绿素含量相关的QTL,位于第19号染色体,可解释表型变异的21.25%,检测到2个与单叶光合速率相关的QTLs,分别位于第9、20号染色体,可解释表型变异的8.39%、14.52%。【结论】检测到与陆地棉叶绿素含量、光合速率性状相关的QTL标记位点,有利于新疆陆地棉高光效辅助选择育种,为进一步提高育种效率打下基础。
【Objective】 The objective of this study is to study the QTL mapping of chlorophyll content and photosynthetic rate in upland cotton in Xinjiang and to provide theoretical guidance for high light efficiency molecular marker assisted selection breeding in upland cotton. 【Method】 The F2 and F2: 3 F2 families and F2 families derived from ZX1789 and E563 low light efficiency upland cotton cultivars (ZX1789 × E563) were used as materials. Of chlorophyll content, photosynthetic rate of QTL mapping. 【Result】 A genetic linkage map consisting of 9 linkage groups with an average distance of 14.7 cM and a total length of 587.3 cM was constructed, accounting for 11.6% of the total in cotton genome. One QTL associated with chlorophyll content was detected on chromosome 19, accounting for 21.25% of the phenotypic variance. Two QTLs associated with leaf photosynthetic rate were detected on chromosomes 9 and 20, 8.39% and 14.52% of phenotypic variation. 【Conclusion】 The detection of QTLs related to chlorophyll content and photosynthetic rate traits of upland cotton is in favor of high light efficiency aided selection breeding of Xinjiang Upland cotton, laying a foundation for further improving the breeding efficiency.