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目的探讨DNA分化抑制因子(inhibitor of DNA differentiation,Id)Id1/Id3在子宫内膜癌中的表达及对子宫内膜癌细胞增殖、侵袭、迁移及粘附等生物学行为的作用。方法 Western blot方法检测4例子宫内膜癌组织及相应的癌旁组织中的Id1/Id3的表达;将子宫内膜癌RL-952和HEC-1-B两株细胞各分为未处理细胞组、空白病毒组、启动子病毒组和Id1/Id3双敲低组。通过qRT-PCR检测不同腺病毒载体感染细胞后MMP2、CXCR4和P21 mRNA表达量,Western blot检测MMP2、CXCR4、P21蛋白表达情况;通过MTT增殖试验、Transwell侵袭实验、细胞划痕实验和细胞粘附实验检测Id1/Id3表达改变后,对子宫内膜癌细胞增殖、侵袭、移行和粘附能力的影响。结果 Id1/Id3在子宫内膜癌组织中较癌旁组织表达增高(P<0.05);双敲低Id1/Id3,子宫内膜癌细胞中MMP2、CXCR4的mRNA水平和蛋白水平明显降低(P<0.05),P21的mRNA水平和蛋白水平则明显升高(P<0.05),RNAi组与其他3组细胞相比均有显著性差异;Id1/Id3双敲低组子宫内膜癌RL-952和HEC-1-B株细胞增殖能力、侵袭能力、迁移能力和粘附能力均抑制(P<0.05)。结论 Id1/Id3在子宫内膜癌患者组织中高表达,并可通过升调MMP2、CXCR4和降调P21的表达促进子宫内膜癌细胞增殖、侵袭、迁移及粘附作用,靶向Id1/Id3治疗可能成为预防和治疗子宫内膜癌的新方案。
Objective To investigate the expression of Id1 / Id3 gene in endometrial carcinoma and its biological effects on the proliferation, invasion, migration and adhesion of endometrial carcinoma cells. Methods Western blot was used to detect the expression of Id1 / Id3 in 4 cases of endometrial carcinoma and its corresponding paracancerous tissues. The two cell lines, RL-952 and HEC-1-B, were divided into untreated group , Blank virus group, promoter virus group and Id1 / Id3 double knockdown group. The expression of MMP2, CXCR4 and P21 mRNA was detected by qRT-PCR and the expressions of MMP2, CXCR4 and P21 were detected by Western blot. The expressions of MMP2, CXCR4 and P21 were detected by MTT assay, Transwell invasion assay, cell scratch assay and cell adhesion assay The effect of Id1 / Id3 expression on the proliferation, invasion, migration and adhesion of endometrial carcinoma cells was detected by experiments. Results The Id1 / Id3 protein expression in endometrial carcinoma was significantly higher than that in paracancerous tissues (P <0.05). The mRNA and protein levels of MMP2 and CXCR4 in double-knocked down Id1 / Id3 cells were significantly decreased (P < 0.05), P21 mRNA and protein levels were significantly increased (P <0.05), RNAi group and other three groups were significantly different; Id1 / Id3 double knockdown group of endometrial cancer RL-952 and The proliferation, invasion, migration and adhesion of HEC-1-B cells were inhibited (P <0.05). Conclusion Id1 / Id3 is highly expressed in the tissue of patients with endometrial carcinoma, and can promote the proliferation, invasion, migration and adhesion of endometrial carcinoma cells by up-regulating the expression of MMP2, CXCR4 and down-regulating P21 and targeting Id1 / Id3 treatment May become a new scheme for the prevention and treatment of endometrial cancer.