目的:观察螺内酯(spironolactone,SL)对大鼠角膜碱烧伤后角膜新生血管(corneal neovascularization,CRNV)形成的抑制作用。方法:健康SD大鼠42只随机分为3组,6只6眼为正常组,其余36只36眼建立碱烧伤诱导的大鼠CRNV模型后随机分为实验组和对照组,每组18只。实验组给予SL(100mg/kg)灌胃,对照组给予等量生理盐水灌胃,1次/d。术后第4,7,14d在裂隙灯显微镜下观察CRNV的面积。结果:对照组在碱烧伤后4d,大鼠角巩膜缘处有新生血管芽成刷状生长,生长速度迅速,至14d新生血管交织成网状,几乎布满整个角膜。实验组大鼠CRNV管径细、分布生长速度缓慢。在碱烧伤后4,7和14d实验组CRNV面积均明显小于对照组(P<0.01)。 Objective: To observe the inhibitory effect of spironolactone (SL) on the formation of corneal neovascularization (CRNV) after corneal alkali burn in rats. Methods: Forty-two healthy SD rats were randomly divided into three groups, six of six were normal group, and the other 36 of 36 were established alkali burns-induced CRNV model rats were randomly divided into experimental group and control group, each group of 18 . The experimental group was given SL (100mg / kg) gavage, the control group was given the same amount of saline gavage once a day. The area of ​​CRNV was observed under slit lamp microscope on the 4th, 7th and 14th day after operation. RESULTS: In the control group 4 days after alkali burns, the neovascular buds grew into a brush-like growth at the sclera of the rats. The growth rate was rapid. The neovascularization was reticulated in the 14th day and covered almost the entire cornea. The CRNV diameter of the rats in the experimental group was small and the growth rate of distribution was slow. At 4, 7 and 14 days after alkali burn, the CRNV area in the experimental group was significantly smaller than that in the control group (P <0.01).