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生物学通报1954年3期刊载了第二军医大学王、胡二氏的脊椎动物脉循环器官着色法,本校在今年准备教学过程中曾按照该法制作一些脉循环器官(动脉)标本。在起初制作中效果不太好。可能是技术上不熟练,常因注射溶液过多,致血管发生破裂,破裂之处多数在主动脉弓上。但若减少分量,则又难以达到注射之目的。总言之,我们难以掌握到适当的分量,不是过多,便是过少。但经采用下面二种改进办法後,效果较好。特介绍如下;(1)按王、胡二氏介绍之法在注射溶液之前从动脉血管中抽出血液30—40cc(愈多愈好)。抽血时最好将兔子麻醉,否则当兔子挣扎时容易刺破血管。抽血後再行注射色剂。因为血管中血量减少了,注射之色剂表现的比较明显。(2)将兔麻醉至死。剖开胸腹腔,剖开时注意不要损伤内脏器官,更不能损伤血管。然後徐徐注入色剂。注射时要注意血管之紧张度,特别是主动脉弓的地方(此处最容易破裂),达到一定程度时即应停止注射。一般在主动脉紧张度达到一定程度时注射之色剂量亦已足够,如色剂不能进人小血管,还可用手按摩引流,或有帮助。
Bulletin of Biology, 1954, No. 3 published the Second Military Medical University Wang, Hu’s vertebrate circulatory organ coloring method, the school this year in preparation for teaching process in accordance with the law made some circulatory organ (artery) specimens. In the initial production effect is not very good. May be technically unskilled, often due to injection solution too much, resulting in rupture of the blood vessels, the rupture of the majority of the aortic arch. However, if the weight is reduced, it is difficult to achieve the purpose of injection. In short, we find it hard to grasp the appropriate weight, not too much or too little. However, after the adoption of the following two improvements, the effect is better. Special introduction is as follows; (1) according to Wang, Hu introduced the law before the injection of blood from the artery in the blood 30-40cc (the more the better). Rabbits anesthetize the best blood, otherwise easy to pierce the blood vessels when the rabbit struggles. After the blood injection injection toner. Because the blood in the blood vessels decreased, the injection of the toner performance is more obvious. (2) The rabbits are anesthetized to death. Cut open the chest and abdomen, cut open, be careful not to damage the internal organs, but can not damage the blood vessels. Then slowly inject the toner. Injections should pay attention to the tension of the blood vessels, especially the aortic arch where the most likely to rupture, to a certain extent, should stop the injection. Generally when the aortic tension reaches a certain level of injection of the amount of color is sufficient, such as the toner can not enter the small blood vessels, but also can be hand massage drainage, or helpful.