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目的:了解T细胞受体信号传导分子CD3γδεζ基因在T和B细胞白血病病人中的表达特点。方法:采用SYBR GreenⅠ荧光定量PCR和相对定量分析法检测32例淋巴细胞肿瘤病人:急性B淋巴细胞白血病(B-ALL)12例、慢性B淋巴细胞白血病(B-CLL)9例、急性T淋巴细胞白血病(T-ALL)11例及10例健康人外周血单个核细胞中CD3各亚单位表达情况,以β2微球蛋白基因(β2M)作为内参,采用公式2-△Ct×100%计算CD3分子γ、δ、ε和ζ链表达水平。结果:B-ALL病人的CD3分子γ、δ和ε链表达中位数水平分别为4.29%,0.67%,17.8%,与健康对照组相比,无显著性差异(P>0.05);而CD3γ链表达水平为0.99%,与健康对照组相比,显著降低(P=0.02)。B-CLL病人的CD3δ、ε基因的表达分别为0.52%,6.72%,与健康对照组相比,无显著性差异(P>0.05);而CD3γ、ζ基因(2.72%,0.77%)则比健康人明显下降(P<0.05)。在T-ALL病人中,CD3分子γ、δ和ε的表达(32.99%,12.9%,61.56%)与健康对照组相比,均明显上升(P<0.05),而CD3ζ的表达量为4.17%,与健康人无显著性差异(P=0.44)。B-ALL、B-CLL病人和健康对照组的CD3分子各链的表达模式依次为CD3ε,CD3γ,CD3ζ,CD3δ,而T-ALL病人的表达模式顺序为CD3ε,CD3γ,CD3δ,CD3ζ。结论:在B细胞白血病中CD3各基因的表达水平多为降低,其变化可能与机体T细胞免疫缺陷相关。而在T细胞白血病中CD3各基因表达水平增加可能是白血病性T细胞一个特点。
Objective: To investigate the expression of T cell receptor signaling molecule CD3γδεζ in patients with T and B cell leukemia. Methods: SYBR Green Ⅰ fluorescence quantitative PCR and relative quantitative assay were used to detect 32 cases of lymphocytic tumor patients: 12 cases of acute B-lymphoblastic leukemia (B-ALL), 9 cases of chronic B-cell lymphocytic leukemia (B-CLL) The expression of CD3 subunit in peripheral blood mononuclear cells of 11 cases of T-ALL and 10 cases of healthy human was calculated by the formula 2-Δ Ct × 100% with β2 microglobulin gene (β2M) Molecular γ, δ, ε and ζ chain expression levels. Results: The median levels of CD3 γ, δ and ε chain in B-ALL patients were 4.29%, 0.67% and 17.8%, respectively, which were not significantly different from those in healthy controls (P> 0.05) The expression level of the chain was 0.99%, which was significantly lower than that of the healthy control group (P = 0.02). The expression of CD3δ and ε genes in patients with B-CLL were 0.52% and 6.72%, respectively, which showed no significant difference compared with healthy controls (P> 0.05). The CD3γ and ζ genes (2.72%, 0.77% Healthy people decreased significantly (P <0.05). The expression of CD3 molecules γ, δ and ε (32.99%, 12.9%, 61.56%) were significantly increased in T-ALL patients compared with healthy controls (P <0.05), while the expression of CD3ζ was 4.17% , No significant difference with healthy people (P = 0.44). The expression patterns of CD3 molecules in B-ALL, B-CLL patients and healthy controls were CD3ε, CD3γ, CD3ζ and CD3δ, respectively. The expression patterns of CD3ε, CD3γ, CD3δ and CD3ζ in T-ALL patients were in order. Conclusion: The expression of CD3 gene in B-cell leukemia is mostly decreased, which may be related to T-cell immunodeficiency. In T-cell leukemia CD3 gene expression levels may be a feature of leukemia T cells.