1996年11月～1997年12月,因超声发现异常,血清筛选异常或高龄孕妇到妇科遗传科进行侵入性产前诊断遗传咨询。904例羊膜穿刺后未培养的羊水细胞进行快速荧光原位杂交(FISH)和标准的核型分析。取2～5 ml清亮羊水于1000转/min,离心5 min进行FISH分析。去上清液后细胞团悬浮于0.56％ KCL 3 ml中,在37℃水浴中20min,加2 ml甲醇/冰醋酸(3:1)。轻轻旋转样本,在1 000转/min下离心悬液5 min。细胞团悬浮在0.5 ml的上述固定液中,此固定标本可在-20℃长期保存或离心调整容量后直接放在载玻片上,制成细胞悬浮液滴 November 1996 ~ December 1997, due to abnormal ultrasound findings, abnormal serum screening or advanced pregnant women to the gynecological genetic department for invasive genetic diagnosis of prenatal counseling. A total of 904 cases of amniotic fluid after amniocentesis were subjected to rapid fluorescence in situ hybridization (FISH) and standard karyotype analysis. Take 2 ~ 5 ml of clear amniotic fluid at 1000 rev / min, centrifugation 5 min FISH analysis. After removing the supernatant, the pellet was suspended in 3 ml of 0.56% KCL and added with 2 ml of methanol / glacial acetic acid (3: 1) in a water bath at 37 ° C for 20 min. Gently rotate the sample and centrifuge the suspension at 1000 rpm for 5 min. The cell pellet is suspended in 0.5 ml of the above fixative solution. The fixative sample can be stored at -20 ° C. for a long time or centrifuged to adjust the capacity and placed directly on a slide to prepare a cell suspension droplet