论文部分内容阅读
表面等离子体共振传感器有可能发展成为一种灵敏的高通量检测的蛋白质组学研究工具,而信号获取和数据处理是其关键之一。本文利用数值模拟全面研究了微阵列相位检测的分辨率与空间采样频率、采样周期数以及ADC位数等关系,详细分析了相关、正弦拟合和傅立叶变换(FTP)等算法对相位检测的精度和误差等影响。研究结果表明,在理想状况下,4个CCD像素就能满足分辨率对光电转换的要求,而在有噪音的情况下,分辨率随着空间采样频率的增加而提高;信号周期数对分辨率影响不大,但有利于克服噪声影响,提高精度;ADC对分辨率影响最大,高分辨率需要选择多位ADC,8位ADC时分辨率约为0.15°,10位时约为0.05°,而12位时约为0.01°。
Surface plasmon resonance sensors may develop into a sensitive high-throughput proteomics research tool, and signal acquisition and data processing is one of the keys. In this paper, the relationship between the resolution of the microarray phase detection and the spatial sampling frequency, the number of sampling cycles and the number of ADC bits has been thoroughly investigated by numerical simulation. The accuracy of the phase detection by the correlation, sine fitting and FTP (Fourier Transform) And errors and other effects. The results show that under ideal conditions, four CCD pixels can meet the resolution requirements of photoelectric conversion, and in the case of noise, the resolution increases with the spatial sampling frequency increases; the number of signal periods on the resolution But it is helpful to overcome the influence of noise and improve accuracy. ADC has the biggest influence on the resolution. High resolution needs to select multiple ADCs. The resolution of 8-bit ADC is about 0.15 ° and about 10 ° when it is about 0.05 °. About 12 at 12 o’clock.