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目的 :研究人肺癌组织中细胞增殖状态、凋亡水平与临床病理生理特征之间的关系 ,探讨肿瘤细胞增殖与凋亡的关系。方法 :应用免疫组化法检测了 16 6例肺癌组织和 37例肺良性病变组织中核增殖相关抗原Ki 6 7的表达水平 ,应用TUNEL法检测其细胞凋亡水平。结果 :(1)肺癌组织中Ki 6 7指数显著高于肺良性病变组织(P <0 0 1) ,而凋亡指数显著低于对照组 (P <0 0 1) ;(2 )肺癌组织中Ki 6 7指数增高与肺癌组织学类型、细胞分化程度显著相关 (P <0 0 1) ,而与患者P -TNM分期、淋巴结转移程度、吸烟与否无明显关系 (P >0 0 5 ) ;(3)肺癌组织中凋亡指数与组织学类型、细胞分化程度无关 (P >0 0 5 ) ,而与患者P -TNM分期、淋巴结转移状态显著相关 (P <0 0 1) ;(4)在Ⅰ、Ⅱ期病例中肺癌细胞凋亡指数与Ki 6 7指数呈显著正相关 (RR =0 2 14,P <0 0 5 ) ,而在Ⅲ、Ⅳ期病例中没有发现相关 (P >0 0 5 )。结论 :Ki 6 7指数反映肺癌细胞固有的增殖能力 ,可能与组织学类型及细胞分化程度密切相关 ,而与患者病期无明显关系 ;凋亡指数可能反映机体与肿瘤细胞的相互作用 ,与患者病期及淋巴结转移状态密切相关。
Objective: To study the relationship between cell proliferation, apoptosis and clinical pathophysiological characteristics in human lung cancer tissues, and to explore the relationship between tumor cell proliferation and apoptosis. METHODS: Immunohistochemical method was used to detect the expression of Ki-67 in 166 lung cancer tissues and 37 benign pulmonary lesions. TUNEL method was used to detect the apoptosis level. Results: (1) Ki 67 index in lung cancer tissue was significantly higher than that in benign lung disease tissue (P <0 01), while apoptosis index was significantly lower than that in control group (P <0 01); (2) in lung cancer tissues. The Ki Ki index increased significantly correlated with the histological type and differentiation of lung cancer (P < 0.01), but it had no significant relationship with P-TNM stage, lymph node metastasis, smoking or not (P > 0.05). (3) The apoptotic index was not related to the histological type and degree of cell differentiation in lung cancer (P > 0.05), but was significantly associated with P-TNM stage and lymph node metastasis (P <0 01); (4) There was a significant positive correlation between the apoptotic index of lung cancer and the Ki 67 index in stage I and II cases (RR = 02-14, P <0 05), but no correlation was found in stages III and IV (P > 0). 0 5 ). Conclusion: The Ki 67 index reflects the inherent proliferative capacity of lung cancer cells, which may be closely related to the histological type and degree of cell differentiation, but not to the patient’s disease stage. The apoptotic index may reflect the interaction between the body and tumor cells, and the patient The stage of disease and lymph node metastasis are closely related.