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目的探讨姜黄素对过氧化氢(H2O2)刺激下的乳鼠心肌细胞是否具有作用。方法培养乳鼠心肌细胞,建立H2O2损伤模型,姜黄素组按12.5、25、50μmol/L三种浓度给药,分别测定各组心肌细胞存活率及乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)活性;分析心肌细胞凋亡情况。结果除低剂量姜黄素组外,其它剂量姜黄素组均能显著地提高H2O2所致心肌细胞损伤的心肌存活率(P<0.05);所有剂量姜黄素组均能减少H2O2损伤的心肌细胞LDH和MDA的生成,提高SOD的活性(P<0.05);与H2O2损伤无姜黄素对照组比较,50μmol/L姜黄素组心肌细胞凋亡率显著下降(P<0.01)。结论姜黄素对H2O2损伤的心肌细胞具有保护作用,并呈剂量依赖性,其作用机制可能与姜黄素清除氧自由基有关。
Objective To investigate whether curcumin has effects on hydrogen peroxide (H2O2) stimulated neonatal rat cardiomyocytes. Methods The cultured neonatal rat cardiomyocytes were established and the H2O2 injury model was established. The curcumin group was administered at concentrations of 12.5, 25, and 50 μmol/L. The viability and lactate dehydrogenase (LDH) and malondialdehyde (MDA) were measured in each group. MDA) and superoxide dismutase (SOD) activity; analysis of cardiomyocyte apoptosis. RESULTS: In addition to the low-dose curcumin group, other doses of curcumin group could significantly increase the myocardial viability of H2O2-induced cardiomyocyte injury (P<0.05); all doses of curcumin group could reduce the H2O2-induced damage of cardiomyocyte LDH and The production of MDA increased the activity of SOD (P<0.05). Compared with the curcumin-free control group of H2O2 injury, the apoptosis rate of cardiomyocytes in the 50μmol/L curcumin group decreased significantly (P<0.01). Conclusion Curcumin has a protective effect on H2O2-injured cardiomyocytes in a dose-dependent manner. Its mechanism may be related to curcumin scavenging oxygen free radicals.