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目的 了解狼疮肾炎 (LN)患者外周血单个核细胞 (PBMCs)白细胞介素 6 (IL 6 )与IL 6受体 (gp80与gp130 )的mRNA表达情况 ,并分析它们之间及其与自身抗体的关系。 方法 采用逆转录聚合酶链反应 (RT PCR)方法检测 18例活动期LN患者、16例非活动期LN患者和 10名正常人PBMCs内IL 6、gp80与 gp130的mRNA表达水平。 结果 ①活动期LN患者、非活动期LN患者及正常人PBMCsIL 6mRNA检出阳性率各为 72 2 %、37 5 %和 10 0 %,前者显著高于后两者 ,而后两者PBMCsIL 6mRNA的检出阳性率差异则无显著性 ;这三组人群PBMCs内gp80mRNA与 gp130mRNA的检出阳性率均无统计学差异。②活动期LN患者PBMCs中IL 6mRNA表达水平高于非活动期LN患者 ,后者又高于正常人 ;活动期LN患者PBMCs中有较高水平的gp80mRNA与 gp130mRNA表达 ,而非活动期LN患者PBMCsgp80mRNA与 gp130mRNA表达水平 ,与正常对照比较则无统计学差异。③活动期LN患者PBMCs内IL 6mRNA、gp80mRNA和gp130mRNA相对表达水平互呈正相关 ,但在非活动期LN患者中则未见这种正相关关系。活动期LN患者PBMCs内IL 6、gp80和gp130基因表达水平与血清ANA、抗ds DNA抗体和抗Sm抗体均无显著的相关性。 结论 活动期LN患者PBMCs内IL 6及其受体gp80和 gp130的mRNA表达均上调 ,且受体 g
Objective To investigate the mRNA expression of interleukin - 6 (IL 6) and interleukin 6 receptor (gp80 and gp130) in peripheral blood mononuclear cells (PBMCs) from patients with lupus nephritis (LN) and analyze their relationship with autoantibodies relationship. Methods The mRNA expression levels of IL 6, gp80 and gp130 in 18 active LN patients, 16 inactive LN patients and 10 normal human PBMCs were detected by reverse transcriptase polymerase chain reaction (RT PCR). Results ① The positive rates of IL 6 mRNA in active LN patients, non-active LN patients and normal PBMCs were 72 2%, 37 5% and 100% respectively, the former was significantly higher than the latter two, and the positive rate of IL 6 mRNA The positive rate difference was not significant; these three groups of PBMCs gp80mRNA and gp130mRNA positive rate was no significant difference. ② The level of IL-6 mRNA in PBMCs of patients with active LN was higher than that of patients with inactive LN, which was higher than that of normal subjects. The levels of gp80mRNA and gp130mRNA in PBMCs of active LN patients were significantly higher than that in inactive LN patients And gp130 mRNA expression levels, compared with the normal control no significant difference. ③ The relative expression levels of IL 6 mRNA, gp80 mRNA and gp130 mRNA in PBMCs of LN patients during active phase were positively correlated with each other, but no positive correlation was observed in inactive LN patients. The levels of IL-6, gp80 and gp130 in PBMCs of active LN patients were not significantly correlated with serum ANA, anti-ds DNA antibody and anti-Sm antibody. Conclusion The mRNA expressions of IL-6 and its receptors gp80 and gp130 in PBMCs of active LN patients were up-regulated, and the receptor g