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[Objective] The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province. [Method] The root tip of A.macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [Result] Allium macrostemon Bunge in Laizhou City introduced in this study was triploid; its somatic chromosome number was 24 and karyotype formula was K(2n)=3x=24m(2SAT)+1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells. [Conclusion] This introduction of triploid A.macrostemon found in China was the first time.
[Objective] The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province. [Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [Result] Allium macrostemon Bunge in Laizhou City introduced in this study was triploid; its somatic chromosome number was 24 and karyotype formula was K (2n ) = 3x = 24m (2SAT) + 1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and the chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells. [Conclusion] This introduction of triploid A. macrostemon found in China was the first time.