莨菪碱和酸性染料溴甲酚紫在pH5.8磷酸盐缓冲液中形成的络合物可溶于氯仿，并在420nm波长处具有最大的吸收，采用酸性染料比色法测定了颠茄合剂中莨菪碱的含量，其平均回收率为100.21％，CV为0.61％、方法简单，快速准确、灵敏度高。实验结果表明，含碳酸氢纳的颠茄合剂不稳定，莨菪碱的含量随时间的延长显著下降。 The complex formed between hyoscyamine and acid dye bromocresol purple in pH 5.8 phosphate buffer was soluble in chloroform and had the highest absorbance at the wavelength of 420 nm. Scopolamine content, the average recovery was 100.21%, CV was 0.61%, the method is simple, fast and accurate, high sensitivity. The experimental results show that the mixture containing sodium bicarbonate belladonna instability, the content of scopolamine significantly decreased over time.