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【目的】分析新疆糜子种质资源遗传多样性和亲缘关系,为选育糜子优质种质资源提供理论依据。【方法】采用ISSR分子标记,对来自新疆3个地区的25份糜子样品的遗传多样性进行分析,并与来自陕西的5份样品进行比较,探讨其亲缘关系。【结果】用筛选出的13条多态性高、重复性好的引物,分别对供试材料的基因组进行扩增,共获得147条清晰可辨的谱带,其中多态性带134条;多态性比率为91.16%,Nei’s基因多样性指数为0.195,平均Shannon信息指数为0.323,遗传分化系数Gst=0.255 7;采用UPGMA法对4个不同来源的糜子材料的聚类分析表明,新疆的糜子聚为一支。【结论】研究材料的遗传变异主要存在于群体内,来自新疆的糜子材料与来自陕西的存在一定的遗传分化。研究从分子水平上为加强对糜子种质资源的挖掘、优良品种的选育及杂交亲本的选配等提供参考依据。
【Objective】 The genetic diversity and genetic relationship of Xinjiang Millet germplasm resources were analyzed to provide the theoretical basis for breeding elite millet germplasm resources. 【Method】 The ISSR markers were used to analyze the genetic diversity of 25 samples of millet from three regions in Xinjiang and compared with 5 samples from Shaanxi Province to explore their genetic relationship. 【Result】 The results showed that there were 147 clearly identifiable bands with 13 polymorphic bands with 134 polymorphic bands amplified by using the 13 highly polymorphic and reproducible primers. The polymorphism rate was 91.16%, Nei’s gene diversity index was 0.195, the average Shannon’s information index was 0.323, and the genetic differentiation coefficient was Gst = 0.255 7. Cluster analysis of four different sources of millet by UPGMA showed that Xinjiang Millet gather for one. 【Conclusion】 The genetic variation of the materials mainly exists in the population. The material of the millet from Xinjiang and the genetic differentiation from Shaanxi Province. At the molecular level, this study provides references for strengthening the exploitation of the germplasm resources of Milletia miltiorrhiza, the breeding of elite varieties and the selection of hybrid parents.