对国内首次研制的多价钩端螺旋体外膜疫苗接种人体安全性和免疫效果进行研究。 10 73名易感青少年随机接种钩端螺旋体三价 (含黄疸出血群赖型、七日热群七日热型、流感伤寒群流感伤寒型 )外膜疫苗、五价 (含黄疸出血群赖型、七日热群七日热型、流感伤寒群流感伤寒型、秋季热群秋季热型、犬群犬型 )外膜疫苗、菌体疫苗和安慰剂 ,分组进行全身和局部的反应观察 ,并以显微镜凝集试验测定各型抗体。结果显示各疫苗组局部和全身反应轻微。三价和五价外膜疫苗免后 1个月和 3个月抗体滴度高于同期相应菌体疫苗诱导的同型抗体滴度 ;且免后 3个月抗体阳转率也较高。提示外膜疫苗较菌体疫苗有更好的免疫原性。 To study the safety and immune effect of multivalent Leptospiral extracorporeal membrane vaccine inoculated in China for the first time. 10 73 susceptible adolescents were randomly assigned to the leptospiral trivalent (including jaundice hemorrhagic group Lai, seven days heat group on the 7th fever, influenza typhoid fever group) outer membrane vaccine, pentavalent (including jaundice bleeding group , Seven-day fever group on the 7th heat-type, influenza typhus group typhus, autumn heat group autumn heat type, canine dog type) outer membrane vaccine, bacterial vaccine and placebo, group systemic and local response observation, and Each type of antibody was determined by microscope agglutination test. The results showed that the local and systemic reactions were mild in each vaccine group. Antibody titer of trivalent and pentavalent outer membrane vaccine at 1 month and 3 months after vaccination was higher than that of the corresponding cell vaccine induced by the corresponding cell vaccine in the same period; and antibody positive rate was also higher 3 months after vaccination. Tip outer membrane vaccine than the bacterial vaccine has better immunogenicity.