我们用4-氨基安替比林(4-AAP)作底物显色剂做ELISA,获得了满意结果.介绍如下.1 4-AAP 溶液称取苯酚(国营练湖化工厂生产,A.R.)550mg,4-AAP(上海试剂一厂,A.R.)24mg,溶于100 ml 0.1 mol/L pH7.4磷酸盐缓冲液 We used 4-aminoantipyrine (4-AAP) as a substrate reagent ELISA, obtained satisfactory results are described below .1 4-AAP solution weighed phenol (State Lake practice Lake Chemical Plant, AR) 550mg , 4-AAP (Shanghai Reagent Factory, AR) 24mg, dissolved in 100ml 0.1mol / L pH7.4 phosphate buffer